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KMID : 0355519920160020131
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Korean Journal of Oral Biology
1992 Volume.16 No. 2 p.131 ~ p.138
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Abstract
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It has been reported that the composition of dental implant might be associated with biocompatibility. The purpose of this study is to know the in vivo cytotoxicity of blade implant and the effects of blade implant on fibroblast in vitro.
50ul. 100ul and 150ul of the cultured supernatant of blade implant were injected into mouse for in vivo cytotoxicity of blade implant and were added to cultured fibroblast. The cell activity of cultured supernatant of blade implant were measured
by
MTT
assay.
In in vivo cytotoxicity test. mild muscular destruction by inflammatory cells was observed in 100ul injected group compared to 50ul injected group. Severe interstitial inflammatory cell infiltration in subcutaneous tissue was observed in 100 and
150ul
injected group compared to 50ul injected group. Moderate interstitial edema was showed in 50 and 100ul injected grcup compared to mild interstitial edema of 150ul injected group. Rare hemorrhage and mild interstitial fibrosis was observed
irrespective
of the amount of injection.
In in vitro cell activity test, the cell activity in the presence of 100ul of the cultured supernatant was more than that of 150ul of the cultured supernatant. The difference of cell activity was diminished after two successive washing.
These results suggest that in vivo cytotoxicity might have a positive correlation to in vitro cell activity.
Further study is needed to analyze the cultured supernatant hy HPLC.
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